Today animal fat and food high in cholesterol notably eggs are the major constituents of our daily diet that increases the blood cholesterol level and lead to cardio vascular diseases. Cardio-vascular diseases and diabetes are the major causes of mortality in many developed countries including India. So that to reduce the risk of cardio vascular diseases and diabetes I have conducted this experiment to see the effect of garlic oil on performance, blood glucose, serum and egg yolk lipid profile in birds. The study was conducted on a total of 24 healthy birds of 40 weeks of age. Birds were procured from AICRP, Adhartal, Jabalpur, India. Birds were randomly divided into 4 groups (T1, T2, T3 and T4)containing 6 birds in each group. Maximum reduction in blood glucose, serum and egg yolk lipid profile was reported in the T4 group.It is recommended that the garlic oil at a dose rate of 750mg/kg of feed was the most effective doses for reducing lipid contents and increases egg production can be effectively used as a dietary supplement in poultry.