The aim of study was to evaluate plasma antioxidant status, protein content, glycemic level, lipid profiles and lipid peroxidation products in type 2 diabetic patients and any effect of gender on these biochemical parameters. Plasma total cholesterol (TC), triglycerides (TG), low density lipoproteins (LDL) and high density lipoproteins (HDL) levels, Antioxidants i.e. superoxide dismutase (SOD, EC 18.104.22.168), catalase (CAT, EC 22.214.171.124) peroxidase (POD, EC 126.96.36.199) and plasma Malondialdehyde (MDA) content were significantly higher in diabetic patients. Elevated glucose level, lipid profiles and peroxidation products indicating oxidative stress damage in diabetic patients. Antioxidants like SOD and CAT were enhanced in response to oxidative stress by hyperglycemia and thus to detoxify the excessive reactive oxygen species (ROS).