During the course of history, microorganisms have spread deadly diseases and caused widespread epidemics that threatened and altered human civilization. Bovine Mastitis is an inflammation of the memory glands of dairy cows accompanied by physical, chemical, pathological and bacteriological changes in milk and glandular tissue. It is the most common infectious diseases in dairy animals in the world which affects the dairy industry. Coagulase Negative Staphylococcus (CNS) is one of the major causative agents to the Bovine Mastitis. The work was carried out on selected herds located in Dharwad district, Karnataka, India to determine the infection status of cows by microbiological analysis of aseptically taken milk samples. This area has numerous smallholdings (farms) and a small number of larger herds. The antimicrobial susceptibility tests carried out in the work indicated the existence of resistance against one or more antibiotics in CNS.