The emergence and spread of antibiotic resistance pathogen is an alarming concern in clinical practice. Many organisms such as MRSA, HIV-1, Hepatitis-B Virus, and Ampicillin resistant E.coli are difficult to treat. There is a need of a cheap broad-active agent that can be used against variety of pathogen. The AgNPs have been found to be effective against many viruses and bacterial species. The use of noble metals at nano-sizes to treat many conditions is gaining importance. The recent development in nanotechnology has pro-vided tremendous impetus in this direction due to its capacity of modulating metals into nanosizes and various shapes, which drastically changes the chemical, physical and optical properties and their use. The efficacy of AgNPs against HIV-1 has been reported by many laboratories including ours [19,26]. It has been shown that AgNPs have got anti-HIV-1 activity and can help the host immune system against HIV-1. This has laid ground for the development of new, potent antiviral drugs capable of preventing HIV infection and controlling virus replication. Recently, it has been demonstrated That AgNPs function as broad-spectrum virucidal and bactericidal agents.